概述
在實(shí)際的蛋白分離、分析工作中，廣大科研工作者經(jīng)常會(huì)遇到某些疏水性蛋白及聚合物改性蛋白在常規(guī)SEC柱上出峰狀態(tài)與理論不符的情況。針對(duì)此種情況，賽分科技在SRT系列色譜柱的基礎(chǔ)上，專門推出針對(duì)該類樣品的Sepax SRT-C SEC系列色譜柱。該鍵合固定相采用的表面修飾技術(shù)，通過(guò)在高純度的硅膠基質(zhì)上鍵合均勻的納米厚度中性親水涂層而制備得到。由于采用可控的化學(xué)修飾技術(shù)，確保了填料批間有著可靠的重現(xiàn)性。SEC填料采用化學(xué)鍵合技術(shù)，表面親水涂層覆蓋完全，因此不僅具有優(yōu)異的穩(wěn)定性，而且對(duì)蛋白等生物樣品的非特異性吸附作用也非常小。同時(shí)，大的孔體積保證了高的載樣量及優(yōu)異的分辨率。
SRT-C體積排阻色譜柱優(yōu)點(diǎn)
高載量及高分辨率 穩(wěn)定的批間重現(xiàn)性 高的蛋白回收率，且蛋白不易失活 極低的非特異性吸附 專為疏水性蛋白及改性蛋白（如PEG化抗體） 適用于常規(guī)生物樣品的分離與制備。技術(shù)參數(shù)：
| 固定相 | SRT-C SEC-150 | SRT-C SEC-300 | SRT-C SEC- 500 |
| 材料 | Neutral, hydrophilic film bonded silica | Neutral, hydrophilic film bonded silica | Neutral, hydrophilic film bonded silica |
| 顆粒大小 | 5 mm | 5 mm | 5 mm |
| 孔徑 (Å) | ~ 150 | ~ 300 | ~ 500 |
| 蛋白質(zhì)分子量范圍 | 500 - 150,000 | 5,000 – 1,250,000 | 15,000 – 5,000,000 |
| 水溶性聚合物分子量范圍 | 500-25,000 | 1,000-100,000 | 25,000-500,000 |
| pH 穩(wěn)定性 | 2 – 8.5 (pH 8.5-9.5 can be tolerated temporarily.) | 2 – 8.5 (pH 8.5-9.5 can be tolerated temporarily.) | 2 – 8.5 (pH 8.5-9.5 can be tolerated temporarily.) |
| 反壓(psi for a 7.8x300 mm) | ~ 700 | ~ 700 | ~ 700 |
| 壓力 (psi) | ~ 4,500 | ~ 3,500 | ~ 3,000 |
| 鹽濃度范圍 | 20 mM - 2.0 M | 20 mM - 2.0 M | 20 mM - 2.0 M |
| 使用溫度 (oC) | ~ 80 | ~ 80 | ~ 80 |
| 流動(dòng)相兼容性 | Aqueous and organic | Aqueous and organic | Aqueous and organic |
訂購(gòu)信息Sepax SRT-C SEC-150
| 產(chǎn)品名稱 | 粒度 | 孔徑 | 規(guī)格 | 訂貨號(hào) |
| SRT-C SEC-150 | 5um | 150 Å | 21.5x600mm | 235150-21560 |
| SRT-C SEC-150 | 5um | 150 Å | 21.5x300mm | 235150-21530 |
| SRT-C SEC-150 | 5um | 150 Å | 7.5x600mm | 235150-7560 |
| SRT-C SEC-150 | 5um | 150 Å | 7.8x300mm | 235150-7830 |
| SRT-C SEC-150 | 5um | 150 Å | 7.8x150mm | 235150-7815 |
| SRT-C SEC-150 | 5um | 150 Å | 7.8x50 (Guard) | 235150-7805 |
| SRT-C SEC-150 | 5um | 150 Å | 4.6x300mm | 235150-4630 |
| SRT-C SEC-150 | 5um | 150 Å | 4.6x150mm | 235150-4615 |
| SRT-C SEC-150 | 5um | 150 Å | 4.6x50 (Guard) | 235150-4605 |
Sepax SRT-C SEC-300
| 產(chǎn)品名稱 | 粒度 | 孔徑 | 規(guī)格 | 訂貨號(hào) |
| SRT-C SEC-300 | 5um | 300 Å | 21.5x600mm | 235300-21560 |
| SRT-C SEC-300 | 5um | 300 Å | 21.5x300mm | 235300-21530 |
| SRT-C SEC-300 | 5um | 300 Å | 7.5x600mm | 235300-7560 |
| SRT-C SEC-300 | 5um | 300 Å | 7.8x300mm | 235300-7830 |
| SRT-C SEC-300 | 5um | 300 Å | 7.8x150mm | 235300-7815 |
| SRT-C SEC-300 | 5um | 300 Å | 7.8x50 (Guard) | 235300-7805 |
| SRT-C SEC-300 | 5um | 300 Å | 4.6x300mm | 235300-4630 |
| SRT-C SEC-300 | 5um | 300 Å | 4.6x150mm | 235300-4615 |
| SRT-C SEC-300 | 5um | 300 Å | 4.6x50 (Guard) | 235300-4605 |
Sepax SRT-C SEC-500
| 產(chǎn)品名稱 | 粒度 | 孔徑 | 規(guī)格 | 訂貨號(hào) |
| SRT-C SEC-500 | 5um | 500 Å | 21.5x600mm | 235500-21560 |
| SRT-C SEC-500 | 5um | 500 Å | 21.5x300mm | 235500-21530 |
| SRT-C SEC-500 | 5um | 500 Å | 7.5x600mm | 235500-7560 |
| SRT-C SEC-500 | 5um | 500 Å | 7.8x300mm | 235500-7830 |
| SRT-C SEC-500 | 5um | 500 Å | 7.8x150mm | 235500-7815 |
| SRT-C SEC-500 | 5um | 500 Å | 7.8x50 (Guard) | 235500-7805 |
| SRT-C SEC-500 | 5um | 500 Å | 4.6x300mm | 235500-4630 |
| SRT-C SEC-500 | 5um | 500 Å | 4.6x150mm | 235500-4615 |
| SRT-C SEC-500 | 5um | 500 Å | 4.6x50 (Guard) | 235500-4605 |
注：以上為常備規(guī)格訂貨信息，其它規(guī)格或特殊定制產(chǎn)品，電詢。
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