重組小鼠 Legumain/ LGMN 蛋白 (His標簽)

蛋白名稱：Mouse Legumain/ LGMN Protein (His Tag)  
蛋白構建：A DNA sequence encoding the extracellular domain (Val 18-Tyr 435) of mouse LGMN (NP_035305.1) precursor was expressed with a C-terminal polyhistidine tag.  
表達宿主：HEK293 Cells  
蛋白純度：≥ 75 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.  
蛋白活性：Measured by its ability to cleave the fluorogenic peptide substrate, N-carbobenzyloxy-Ala-Ala-Asn-7-amido-4-methyl coumarin(Z-AAN-AMC). The specific activity is > 350 pmoles/min/μg. (Activation description: The enzyme achieves its activity under acidic pH)  
蛋白內毒素：< 1.0 EU per μg of the protein as determined by the LAL method  
預測N端：Thr 22  
蛋白分子量：The secreted recombinant mouse LGMN consists of 434 amino acids and has a calculated molecular mass of 49.8 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 55 kDa protein in SDS-PAGE under reducing conditions.  
蛋白NP號：NP_035305.1  
蛋白氨基酸序列：Val18-Tyr435  
蛋白標簽：N-His

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