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公司基本資料信息
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美國Advanced BioMatrix是3D組織培養、細胞檢測和細胞增殖等領域實驗解決方案的佼佼者。Advanced BioMatrix在分離、純化、凍干、細胞培養和蛋白檢測、多肽粘附、附著因子、基質硬度和其他3D matrix 產品開發方面有著豐富的經驗。Advanced BioMatrix的研發經驗和專業知識確保其產品可達到質量，并保證產品之間一致性，方便研究客戶使用。
以下為Advanced BioMatrix3D Matrices 產品競爭優勢：
1. 提供高純度和成分確定的胞外基質；
2. 超過1000余篇文獻引用PureCol產品，品質非常均一；
3. 在3D培養基領域可提供全面的產品線；
4. 可提供特異性剛性硅基板的公司（CytoSoft）;
5. 可提供可溶性絲纖蛋白的供應商（可運用于多種3D培養）；
6. 如果客戶接觸3D膠原凝膠，Advanced BioMatrix還是的預制膠原蛋白(PureCol EZ Gel)供應商；
以下產品為Advanced BioMatrix暢銷品：
1. PureCol 牛源I型膠原蛋白 3 mg/ml #5005-100ML
2. Nutragen牛源I型膠原蛋白 6 mg/ml #5010-50ML
3. FibriCol 牛源I型膠原蛋白 10 mg/ml #5133-20ML
4. VitroCol 人源I型膠原蛋白 #5007-20ML
5. 彈性蛋白原 #5052-1MG
6. ECM Selec Array kit Ultra-36 #5170-1EA
7. CytoSoft（剛性可變的基底，Advanced BioMatrix添加產品5190-7EA）
8. 人III型膠原蛋白 #5021-10MG
9. 人IV型膠原蛋白 #5022-5MG
10. Silk Fibroin溶液 #5154-20ML
11. Fibronectin #5080-5MG
12. Vitronectin #5051-0.1MG
Advanced BioMatrix暢銷的產品是PureCol（5005-100ML）。目前已有上千發表的論文使用這款產品。（Nutragen 5010、fibricol 5133和PureCol的物質成分一樣，僅僅是濃度不同）濃度越高，膠原蛋白硬度越強。
許多科研人員正從Matrigel產品使用習慣轉換過來，下面簡單比較下Advanced BioMatrix公司的PureCol和Matrigel：
| Matrigel | PureCol |
| 濃度不均一 | 均一且濃度精確 |
| 鼠，來源 | 牛，非來源 |
| 未知組分 | 已知且明確的組分 |
| 需在冰上分裝操作 | 簡單易用 |
客戶可從上表對比Matrigel和VitroCol （人I型膠原蛋白），以人源簡單代替牛源。
PureCol® EZ Gel--3D膠原凝膠讓實驗更簡單
含牛源I型膠原蛋白（5 mg/ml）的DMEM/F-12 培養基, 并添加谷氨酰胺，中性pH。
商品說明：PureCol® collagen is known as the standard of all collagens for purity (>99.9% collagen content), functionality, and the most native-like collagen available. PureCol® is isolated from bovine hides sourced from the only controlled, closed herd in the United States. Advanced BioMatrix’s manufacturing processes comply with stringent quality standards that have proven to yield unsurpassed lot-to-lot consistency.
PureCol® collagen is approximately 97% Type I collagen with the remainder being comprised of Type III collagen. It contains a high monomer content as measured by gel permeation chromatography. PureCol® collagen is supplied at approximately a 3 mg/ml concentration. The concentration for each specific lot is provided on a Certificate of Analysis that is available with the purchase of each product. PureCol® is soluble atelo-collagen in 0.01 N HCI, therefore, the pH is approximately 2.0. PureCol® collagen is ideal for coating of surfaces, providing preparation of thin layers for culturing cells, or use as a solid gel.
PureCol® collagen is provided in a user-friendly packaging for use and storage. PureCol® is sterile filtered and is supplied as a ready to use solution.
Purecol膠原蛋白被稱為標準純度的膠原蛋白（>99.9%膠原蛋白含量），其功能可媲美大多數的原生膠原蛋白。Purecol提取于來自美國封閉式牛群的牛皮，生物生產過程符合嚴格的質量標準，已被證明是質量超凡的。Purecol膠原蛋白是由約97%的I型膠原蛋白，其余的為III型膠原蛋白組成的，它具有很高的單體含量可以用于測試凝膠滲透色譜。本產品的濃度約為3mg/ml，每個批次的具體濃度會寫于產品的COA上隨貨發出。PureCol是溶于0.01N鹽酸的端膠原蛋白，因此它的PH值約為2。PureCol膠原蛋白是理想的蛋白質涂層，可用于準備培養的細胞或者固體凝膠。PureCol膠原蛋白的包裝便于儲存和使用，經過滅菌的溶液隨時可以使用。
| 產品信息 | PureCol® 膠原蛋白溶液 貨號 # BM-5005-100ML |
| 滅菌方法 | 過濾 |
| 形態 | 溶液 |
| 包裝尺寸 | 100 ml |
| 儲存溫度 | 2-10 °C |
| 有效期 | 詳見產品包裝與隨貨發出的COA |
| 濃度（縮二脲蛋白測定） | 2.9-3.2 mg/ml |
| 純度（膠原蛋白濃度） | > 99.9% |
| pH | pH約為2, 0.01 N HCl |
| 凝膠時間（凝膠時間測定法） | < 40 分鐘 |
| 原纖維形成（纖維形成） | >0.5 吸光度單位 |
| 聚丙烯酰胺凝膠電泳（SDS–PAGE） | > 85%含膠原蛋白在α，β，和γ帶，< 15%含比α帶快的膠原蛋白 |
| 無菌的 | 沒有細菌生長 |
| 內毒素 (LAL) | < 1.0 EU/ml |
| 滲透壓 | ~ 20 mOsm |
| 電導率 | ~ 2300 µs/cm |
| 細胞附著實驗 | 通過 |
| 來源 | 牛皮–胃蛋白酶提取 |
使用方法：
Coating Procedure
Note:Use these recommendations as guidelines to determine the optimal coating conditions for your culture system.
Remove required quantity of collagen from the bottle and dispense into a dilution vessel. Dilute PureCol® in water to ~50 to 100 µg/ml (~1:30). A 0.01 M HCl solution may also be used. Swirl contents gently until material is completely mixed. Add appropriate amount of diluted PureCol® material to the culture surface ensuring that the entire surface is coated. Incubate at room temperature or 37°C, covered, for 1-2 hours. After incubation, aspirate any remaining material. Rinse coated surfaces carefully with sterile medium or PBS, avoid scratching surfaces. Coated surfaces are ready for use. They may also be stored at 2-8°C damp or air dried if sterility is maintained.3-D Gel Preparation Procedure
Slowly add 1 part of chilled 10X PBS or 10X culture media to 8 parts of chilled collagen solution with gentle swirling. Adjust pH of mixture to 7.2–7.6 using sterile 0.1 M NaOH. Monitor pH adjustment carefully (pH meter, phenol red, or pH paper). Adjust final volume to a total of 10 parts with sterile water. To prevent gelation, maintain temperature of mixture at 2–10°C. To form gel, warm to 37°C. Allow approximately 90 to 120 minutes for gel formation.Cell Attachment Bioassay
To demonstrate cell attachment, cells were seeded onto surfaces coated with the matrix product and onto negative control surfaces without the matrix product. Cells were seeded onto surfaces using serum-free DMEM media. All surfaces were blocked with a solution containing 1% BSA. Cells were then allowed to attach for one (1) hour at 37°C. The culture surfaces were washed with DPBS. The results indicate significant cell attachment with surfaces coated using the matrix product.
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