近日,農(nóng)業(yè)微生物資源發(fā)掘與利用全國(guó)重點(diǎn)實(shí)驗(yàn)室、教育部動(dòng)物育種與健康養(yǎng)殖前沿科學(xué)中心、湖北洪山實(shí)驗(yàn)室晏向華教授課題組的研究成果以 “Gut microbiota-derived 3-phenylpropionic acid promotes intestinal epithelial barrier function via AhR signaling”為題在Microbiome雜志發(fā)表。
該研究以貴州從江香豬為研究對(duì)象,發(fā)掘出腸道功能微生物Bacteroides fragilis,并闡明其通過3-苯丙酸代謝物促進(jìn)豬腸道屏障功能的作用機(jī)制,在實(shí)施腸道菌群干預(yù)改善生豬腸道健康方面具有重要意義。
腸道屏障功能是阻止病原體和飼料毒物等有害物質(zhì)進(jìn)入機(jī)體的重要防線,對(duì)維持動(dòng)物機(jī)體內(nèi)穩(wěn)態(tài)具有重要作用。在集約化養(yǎng)豬生產(chǎn)中,早期斷奶措施和換料等應(yīng)激因素可導(dǎo)致豬腸道屏障功能受損,嚴(yán)重影響了腸道對(duì)營(yíng)養(yǎng)物質(zhì)的消化與吸收,同時(shí)增加了腸道受感染的風(fēng)險(xiǎn)。近年來,有研究發(fā)現(xiàn)腸道功能微生物可增強(qiáng)宿主腸道屏障功能,有利于改善腸道健康。因此,深入發(fā)掘可促進(jìn)豬腸道屏障功能的關(guān)鍵微生物并解析其作用機(jī)制具有重要理論意義和應(yīng)用價(jià)值。
研究人員綜合運(yùn)用宏基因組學(xué)和細(xì)菌16S rDNA擴(kuò)增子測(cè)序技術(shù)解析了7個(gè)品種豬(即杜×(長(zhǎng)×大)豬、藏豬、萊蕪豬、沙子嶺豬、從江香豬、環(huán)江香豬和寧鄉(xiāng)豬)共計(jì)56頭斷奶仔豬和56頭育肥末期豬的腸道細(xì)菌區(qū)系組成,發(fā)現(xiàn)不同品種豬的腸道細(xì)菌區(qū)系有差異,其中杜×(長(zhǎng)×大)豬和從江香豬腸道細(xì)菌區(qū)系的差異最為明顯。從江香豬是一種我國(guó)貴州省特有的地方豬種,它具有肉嫩味美、抗病力強(qiáng)、耐粗飼等特點(diǎn)。進(jìn)一步證實(shí)了從江香豬育肥豬的腸道屏障功能比杜×(長(zhǎng)×大)育肥豬更強(qiáng)。將這兩個(gè)品種豬的糞便微生物分別移植到無菌小鼠腸道后,受體無菌小鼠亦表現(xiàn)出差異化的腸道屏障功能特性。通過比較受體無菌小鼠腸道細(xì)菌區(qū)系的差異,篩選并驗(yàn)證出B. fragilis可促進(jìn)宿主腸道屏障功能。進(jìn)一步研究發(fā)現(xiàn)B. fragilis可通過產(chǎn)生代謝產(chǎn)物3-苯丙酸來促進(jìn)宿主腸道屏障功能。功能試驗(yàn)表明3-苯丙酸代謝物可通過激活A(yù)hR信號(hào)增加腸道緊密連接等相關(guān)蛋白的表達(dá),進(jìn)而促進(jìn)腸道屏障功能。綜上,本研究綜合運(yùn)用從江香豬腸道微生物資源,基于糞菌移植、微生物組學(xué)和無菌小鼠等技術(shù)揭示了微生物來源的3-苯丙酸代謝產(chǎn)物促進(jìn)腸道屏障功能的作用機(jī)制,為實(shí)施腸道菌群干預(yù)改善生豬腸道健康提供了依據(jù)。
華中農(nóng)業(yè)大學(xué)動(dòng)物科學(xué)技術(shù)學(xué)院、動(dòng)物醫(yī)學(xué)院博士后胡軍為本文第一作者,晏向華教授為通訊作者。本研究受國(guó)家自然科學(xué)基金、博士后創(chuàng)新人才計(jì)劃、湖北省自然科學(xué)基金、博士后面上項(xiàng)目的資助。
【英文摘要】
Background
The intestinal epithelial barrier confers protection against the intestinal invasion by pathogens and exposure to food antigens and toxins. Growing studies have linked the gut microbiota to the intestinal epithelial barrier function. The mining of the gut microbes that facilitate the function of intestinal epithelial barrier is urgently needed.
Results
Here, we studied a landscape of the gut microbiome of seven pig breeds using metagenomics and 16S rDNA gene amplicon sequencing. The results indicated an obvious difference in the gut microbiome between Congjiang miniature (CM) pigs (a native Chinese breed) and commercial Duroc × [Landrace × Yorkshire] (DLY) pigs. CM finishing pigs had stronger intestinal epithelial barrier function than the DLY finishing pigs. Fecal microbiota transplantation from CM and DLY finishing pigs to germ-free (GF) mice transferred the intestinal epithelial barrier characteristics. By comparing the gut microbiome of the recipient GF mice, we identified and validated Bacteroides fragilis as a microbial species that contributes to the intestinal epithelial barrier. B. fragilis-derived 3-phenylpropionic acid metabolite had an important function on the enhancement of intestinal epithelial barrier. Furthermore, 3-phenylpropionic acid facilitated the intestinal epithelial barrier by activating aryl hydrocarbon receptor (AhR) signaling.
Conclusions
These findings suggest that manipulation of B. fragilis and 3-phenylpropionic acid is a promising strategy for improving intestinal epithelial barrier.
日期:2023-06-13
該研究以貴州從江香豬為研究對(duì)象,發(fā)掘出腸道功能微生物Bacteroides fragilis,并闡明其通過3-苯丙酸代謝物促進(jìn)豬腸道屏障功能的作用機(jī)制,在實(shí)施腸道菌群干預(yù)改善生豬腸道健康方面具有重要意義。
腸道屏障功能是阻止病原體和飼料毒物等有害物質(zhì)進(jìn)入機(jī)體的重要防線,對(duì)維持動(dòng)物機(jī)體內(nèi)穩(wěn)態(tài)具有重要作用。在集約化養(yǎng)豬生產(chǎn)中,早期斷奶措施和換料等應(yīng)激因素可導(dǎo)致豬腸道屏障功能受損,嚴(yán)重影響了腸道對(duì)營(yíng)養(yǎng)物質(zhì)的消化與吸收,同時(shí)增加了腸道受感染的風(fēng)險(xiǎn)。近年來,有研究發(fā)現(xiàn)腸道功能微生物可增強(qiáng)宿主腸道屏障功能,有利于改善腸道健康。因此,深入發(fā)掘可促進(jìn)豬腸道屏障功能的關(guān)鍵微生物并解析其作用機(jī)制具有重要理論意義和應(yīng)用價(jià)值。
研究人員綜合運(yùn)用宏基因組學(xué)和細(xì)菌16S rDNA擴(kuò)增子測(cè)序技術(shù)解析了7個(gè)品種豬(即杜×(長(zhǎng)×大)豬、藏豬、萊蕪豬、沙子嶺豬、從江香豬、環(huán)江香豬和寧鄉(xiāng)豬)共計(jì)56頭斷奶仔豬和56頭育肥末期豬的腸道細(xì)菌區(qū)系組成,發(fā)現(xiàn)不同品種豬的腸道細(xì)菌區(qū)系有差異,其中杜×(長(zhǎng)×大)豬和從江香豬腸道細(xì)菌區(qū)系的差異最為明顯。從江香豬是一種我國(guó)貴州省特有的地方豬種,它具有肉嫩味美、抗病力強(qiáng)、耐粗飼等特點(diǎn)。進(jìn)一步證實(shí)了從江香豬育肥豬的腸道屏障功能比杜×(長(zhǎng)×大)育肥豬更強(qiáng)。將這兩個(gè)品種豬的糞便微生物分別移植到無菌小鼠腸道后,受體無菌小鼠亦表現(xiàn)出差異化的腸道屏障功能特性。通過比較受體無菌小鼠腸道細(xì)菌區(qū)系的差異,篩選并驗(yàn)證出B. fragilis可促進(jìn)宿主腸道屏障功能。進(jìn)一步研究發(fā)現(xiàn)B. fragilis可通過產(chǎn)生代謝產(chǎn)物3-苯丙酸來促進(jìn)宿主腸道屏障功能。功能試驗(yàn)表明3-苯丙酸代謝物可通過激活A(yù)hR信號(hào)增加腸道緊密連接等相關(guān)蛋白的表達(dá),進(jìn)而促進(jìn)腸道屏障功能。綜上,本研究綜合運(yùn)用從江香豬腸道微生物資源,基于糞菌移植、微生物組學(xué)和無菌小鼠等技術(shù)揭示了微生物來源的3-苯丙酸代謝產(chǎn)物促進(jìn)腸道屏障功能的作用機(jī)制,為實(shí)施腸道菌群干預(yù)改善生豬腸道健康提供了依據(jù)。
華中農(nóng)業(yè)大學(xué)動(dòng)物科學(xué)技術(shù)學(xué)院、動(dòng)物醫(yī)學(xué)院博士后胡軍為本文第一作者,晏向華教授為通訊作者。本研究受國(guó)家自然科學(xué)基金、博士后創(chuàng)新人才計(jì)劃、湖北省自然科學(xué)基金、博士后面上項(xiàng)目的資助。
【英文摘要】
Background
The intestinal epithelial barrier confers protection against the intestinal invasion by pathogens and exposure to food antigens and toxins. Growing studies have linked the gut microbiota to the intestinal epithelial barrier function. The mining of the gut microbes that facilitate the function of intestinal epithelial barrier is urgently needed.
Results
Here, we studied a landscape of the gut microbiome of seven pig breeds using metagenomics and 16S rDNA gene amplicon sequencing. The results indicated an obvious difference in the gut microbiome between Congjiang miniature (CM) pigs (a native Chinese breed) and commercial Duroc × [Landrace × Yorkshire] (DLY) pigs. CM finishing pigs had stronger intestinal epithelial barrier function than the DLY finishing pigs. Fecal microbiota transplantation from CM and DLY finishing pigs to germ-free (GF) mice transferred the intestinal epithelial barrier characteristics. By comparing the gut microbiome of the recipient GF mice, we identified and validated Bacteroides fragilis as a microbial species that contributes to the intestinal epithelial barrier. B. fragilis-derived 3-phenylpropionic acid metabolite had an important function on the enhancement of intestinal epithelial barrier. Furthermore, 3-phenylpropionic acid facilitated the intestinal epithelial barrier by activating aryl hydrocarbon receptor (AhR) signaling.
Conclusions
These findings suggest that manipulation of B. fragilis and 3-phenylpropionic acid is a promising strategy for improving intestinal epithelial barrier.
日期:2023-06-13